Journal: iScience

Article Title: Determinants of affinity, specificity, and phase separation in a supramodule from Post-synaptic density protein 95

doi: 10.1016/j.isci.2022.105069

Figure Lengend Snippet:

Article Snippet: Fmoc-L-Asp(tbu)-OH , Gyros Proteintechnologies , SMP-20-DB.

Techniques: Recombinant, Software, Spectrophotometry, Microscopy, Mass Spectrometry, Inverted Microscopy

Journal: iScience

Article Title: Determinants of affinity, specificity, and phase separation in a supramodule from Post-synaptic density protein 95

doi: 10.1016/j.isci.2022.105069

Figure Lengend Snippet:

Article Snippet: Fmoc-L-Arg(pbf)-OH , Gyros Proteintechnologies , SMP-30-RBF.

Techniques: Recombinant, Software, Spectrophotometry, Microscopy, Mass Spectrometry, Inverted Microscopy

Journal: iScience

Article Title: Determinants of affinity, specificity, and phase separation in a supramodule from Post-synaptic density protein 95

doi: 10.1016/j.isci.2022.105069

Figure Lengend Snippet:

Article Snippet: Fmoc-L-Leu-OH , Gyros Proteintechnologies , SMP-30-L.

Techniques: Recombinant, Software, Spectrophotometry, Microscopy, Mass Spectrometry, Inverted Microscopy

Journal: Life

Article Title: Structural Design and Synthesis of Novel Cyclic Peptide Inhibitors Targeting Mycobacterium tuberculosis Transcription

doi: 10.3390/life12091333

Figure Lengend Snippet: Synthetic scheme of (Cyclo-1,6)Ac-CYYQWC-NH 2 with Rink amide MBHA resin: ( a ) Fmoc deprotection: piperidine 20% in DMF; ( b ) addition of Fmoc-L-Cys(Trt)-OH and Fmoc-L-Trp(Boc)-OH. Fmoc-L-Gln(Trt)-OH, Fmoc-L-Tyr(tBu)-OH (2×), Fmoc-L-Cys(Trt)-OH with HCTU/NMM in DMF as the coupling reagent; ( c ) final deprotection with 20% piperidine in DMF, followed by acetylation to the N-terminus with acetic anhydride and 2.6-lutidine in DMF, RT; ( d ) resin cleavage and global deprotection: TFA/TIPS/Phenol/H 2 O (90:5:2.5:2.5), RT, 2 h reaction, followed by crude precipitation and RP-HPLC purification resulted in the linear-Ac-CYYQWC-NH 2 ; and ( e ) cyclization with air dilution in a high dilution of ammonium bicarbonate buffer pH = 8.5, followed by characterization.

Article Snippet: A high-loading Rink amide methylbenzhydrylamine (MBHA) resin, Fmoc-L-Cys(Trt)-OH, Fmoc-L-Trp(Boc)-OH, Fmoc-L-Gln(Trt)-OH, Fmoc-L-Tyr(tBu)-OH, Fmoc-L-Leu-OH, Fmoc-L-His(Trt)-OH, Fmoc-L-Phe-OH, Fmoc-L-Lys(Boc)-OH, Fmoc-L-Val-OH, O-(1H-6-Chlorobenzotriazole-1-yl) (HCTU), and N-methyl morpholine (NMM) were purchased from Gyros Protein Technologies, Tucson, AZ, USA.

Techniques: Purification